Ontology highlight
ABSTRACT:
SUBMITTER: Marras SA
PROVIDER: S-EPMC419623 | biostudies-literature | 2004 May
REPOSITORIES: biostudies-literature
Nucleic acids research 20040520 9
We have developed a simple method to measure RNA synthesis in real time. In this technique, transcription reactions are performed in the presence of molecular beacons that possess a 2'-O-methylribonucleotide backbone. These probes become fluorescent as they hybridize to nascent RNA during the course of synthesis. We found that molecular beacons synthesized from natural deoxyribonucleotides were not suitable, because they are copied by RNA polymerases, generating complementary product strands tha ...[more]