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Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.

ABSTRACT: To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. When stably integrated, the SI-derived grafts show many features unique only to the SI but distinct from the colonic epithelium. Our study provides evidence that cultured adult SI stem cells could be a source for cell therapy of intestinal diseases, maintaining their identity along the gastrointestinal tract through an epithelium-intrinsic mechanism.

SUBMITTER: Fukuda M

PROVIDER: S-EPMC4197962 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.

Fukuda Masayoshi M Mizutani Tomohiro T Mochizuki Wakana W Matsumoto Taichi T Nozaki Kengo K Sakamaki Yuriko Y Ichinose Shizuko S Okada Yukinori Y Tanaka Toshihiro T Watanabe Mamoru M Nakamura Tetsuya T

Genes & development 20140801 16

To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. When stably integrated, the SI-derived grafts show many features unique only to the SI but distinct from the colonic epithelium. Our study provides evidence that ...[more]

PMID: 25128495

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Expression data from heterotopically grafted mouse small intestinal epithelium and the normal colonic epithelium

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2014-08-15 | GSE59410 | GEO

Expression data from heterotopically grafted mouse small intestinal epithelium and the normal colonic epithelium

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