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Development of an immunologically tolerated combination of fluorescent proteins for in vivo two-photon imaging.

ABSTRACT: Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivo imaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic visibility of several commonly used FPs by the murine immune system and identify a spectrally compatible, immunologically tolerated combination of FPs well suited for in vivo two-photon imaging.

SUBMITTER: Gossa S

PROVIDER: S-EPMC4200419 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Development of an immunologically tolerated combination of fluorescent proteins for in vivo two-photon imaging.

Gossa Selamawit S Nayak Debasis D Zinselmeyer Bernd H BH McGavern Dorian B DB

Scientific reports 20141017

Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivo imaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic visibility of several commonly used FPs by the murine immune system and identify a spectrally compatible ...[more]

PMID: 25322934

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