Sensitization of transforming growth factor-? signaling by multiple peptides patterned on DNA nanostructures.
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ABSTRACT:
SUBMITTER: Pedersen RO
PROVIDER: S-EPMC4219643 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
Sensitization of transforming growth factor-β signaling by multiple peptides patterned on DNA nanostructures.
Pedersen Ronnie O RO Loboa Elizabeth G EG LaBean Thomas H TH
Biomacromolecules 20131108 12
We report sensitization of a cellular signaling pathway by addition of functionalized DNA nanostructures. Signaling by transforming growth factor β (TGFβ) has been shown to be dependent on receptor clustering. By patterning a DNA nanostructure with closely spaced peptides that bind to TGFβ receptor, we observe increased sensitivity of NMuMG cells to TGFβ ligand. This is evidenced by translocation of secondary messenger proteins to the nucleus and stimulation of an inducible luciferase reporter a ...[more]
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