Ontology highlight
ABSTRACT:
SUBMITTER: Furman JL
PROVIDER: S-EPMC4227728 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20140530 23
Selective covalent bond formation at a protein-protein interface potentially can be achieved by genetically introducing into a protein an appropriately "tuned" electrophilic unnatural amino acid that reacts with a native nucleophilic residue in its cognate receptor upon complex formation. We have evolved orthogonal aminoacyl-tRNA synthetase/tRNACUA pairs that genetically encode three aza-Michael acceptor amino acids, N(ε)-acryloyl-(S)-lysine (AcrK, 1), p-acrylamido-(S)-phenylalanine (AcrF, 2), a ...[more]