Ontology highlight
ABSTRACT:
SUBMITTER: Dore K
PROVIDER: S-EPMC4230936 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Doré Kim K Labrecque Simon S Tardif Christian C De Koninck Paul P
PloS one 20141113 11
Little is known about the changes in protein interactions inside synapses during synaptic remodeling, as their live monitoring in spines has been limited. We used a FRET-FLIM approach in developing cultured rat hippocampal neurons expressing fluorescently tagged NMDA receptor (NMDAR) and PSD95, two essential proteins in synaptic plasticity, to examine the regulation of their interaction. NMDAR stimulation caused a transient decrease in FRET between the NMDAR and PSD95 in spines of young and matu ...[more]