Ontology highlight
ABSTRACT:
SUBMITTER: Fu X
PROVIDER: S-EPMC4255137 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Fu Xiaofeng X Himes Benjamin A BA Ke Danxia D Rice William J WJ Ning Jiying J Zhang Peijun P
Structure (London, England : 1993) 20141120 12
Cryo-electron tomography (cryoET) has become a powerful tool for direct visualization of 3D structures of native biological specimens at molecular resolution, but its application is limited to thin specimens (<300 nm). Recently, vitreous sectioning and cryoFIB milling technologies were developed to physically reduce the specimen thickness; however, cryoET analysis of membrane protein complexes within native cell membranes remains a great challenge. Here, we use phage ΦX174 lysis gene E to rapidl ...[more]