Ontology highlight
ABSTRACT:
SUBMITTER: Brinkman EK
PROVIDER: S-EPMC4267669 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Brinkman Eva K EK Chen Tao T Amendola Mario M van Steensel Bas B
Nucleic acids research 20141009 22
The efficacy and the mutation spectrum of genome editing methods can vary substantially depending on the targeted sequence. A simple, quick assay to accurately characterize and quantify the induced mutations is therefore needed. Here we present TIDE, a method for this purpose that requires only a pair of PCR reactions and two standard capillary sequencing runs. The sequence traces are then analyzed by a specially developed decomposition algorithm that identifies the major induced mutations in th ...[more]