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Easy quantification of template-directed CRISPR/Cas9 editing.

ABSTRACT: Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE method for quantification of templated editing events, including point mutations. The resulting TIDER method is a rapid, cheap and accessible tool for testing and optimization of template-directed genome editing strategies. A free web tool for TIDER data analysis is available at http://tide.nki.nl.

SUBMITTER: Brinkman EK

PROVIDER: S-EPMC6007333 | biostudies-other | 2018 Jun

REPOSITORIES: biostudies-other

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Easy quantification of template-directed CRISPR/Cas9 editing.

Brinkman Eva K EK Kousholt Arne N AN Harmsen Tim T Leemans Christ C Chen Tao T Jonkers Jos J van Steensel Bas B

Nucleic acids research 20180601 10

Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE method for quantification of templated editing events, including point mutations. The resulting TIDER method is a rapid, cheap and accessible tool for testing and optimization of template-directed genome ...[more]

PMID: 29538768

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