Ontology highlight
ABSTRACT:
SUBMITTER: Suharni
PROVIDER: S-EPMC4278166 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Monoclonal antibodies in immunodiagnosis and immunotherapy 20141201 6
The development of antibodies against human G-protein-coupled receptors (GPCRs) has achieved limited success, which has mainly been attributed to their low stability in a detergent-solubilized state. We herein describe a method that can generally be applied to the selection of phage display libraries with human GPCRs reconstituted in liposomes. A key feature of this approach is the production of biotinylated proteoliposomes that can be immobilized on the surface of streptavidin-coupled microplat ...[more]