Detection of constant domain of human T cell antigen receptor alpha-chain via novel monoclonal antibody 7F18.

ABSTRACT: The ?? T cell antigen receptor (TCR) endows T lymphocytes with immune specificity and controls their effector functions. Each person possesses a vast repertoire of TCRs that is generated by the well-studied processes of somatic recombination and thymic selection. While many antibodies specific for TCR? variable domains are available, antibodies specific for human TCR? are rare. We now report a novel monoclonal antibody, 7F18, which binds to human TCR? constant region, with specificity for a denatured epitope that can be visualized by SDS-PAGE followed by Western blot. Both immature and mature TCR ?-chain products can be visualized, making 7F18 potentially applicable to various biochemical assays of multiprotein complex assembly and maturation. This new monoclonal antibody provides a tool that can potentially facilitate the biochemical analysis of comprehensive populations of human ?? TCR complexes that need not be limited to small subsets of the repertoire.

SUBMITTER: Reed BK 

PROVIDER: S-EPMC4278167 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature