Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide.

ABSTRACT: Structural features of nucleic acids have become an integral part of current biomedical research. Highly selective and readily performed methods with little toxicity that target guanosines in non-duplex nucleic acids are needed, which led us to search for an effective agent for guanosine sequencing. Treatment of DNA or RNA with potassium tungstate and hydrogen peroxide produced damaged guanosines in DNA or RNA sequences. The damaged guanosines in non-duplex DNA could be cleaved by hot piperidine. Similarly, damaged guanosines in non-duplex RNA could be cleaved by aniline acetate. We could identify structural features of nucleic acid using this strategy instead of dimethyl sulphate and Ribonuclease T1.

SUBMITTER: Mao W 

PROVIDER: S-EPMC4288145 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature