Ontology highlight
ABSTRACT:
SUBMITTER: Kuznetsov NA
PROVIDER: S-EPMC4288190 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Kuznetsov Nikita A NA Bergonzo Christina C Campbell Arthur J AJ Li Haoquan H Mechetin Grigory V GV de los Santos Carlos C Grollman Arthur P AP Fedorova Olga S OS Zharkov Dmitry O DO Simmerling Carlos C
Nucleic acids research 20141217 1
Formamidopyrimidine-DNA glycosylase (Fpg) excises 8-oxoguanine (oxoG) from DNA but ignores normal guanine. We combined molecular dynamics simulation and stopped-flow kinetics with fluorescence detection to track the events in the recognition of oxoG by Fpg and its mutants with a key phenylalanine residue, which intercalates next to the damaged base, changed to either alanine (F110A) or fluorescent reporter tryptophan (F110W). Guanine was sampled by Fpg, as evident from the F110W stopped-flow tra ...[more]