Phosphoinositide 3-kinase beta protects nuclear envelope integrity by controlling RCC1 localization and Ran activity.

ABSTRACT: The nuclear envelope (NE) forms a barrier between the nucleus and the cytosol that preserves genomic integrity. The nuclear lamina and nuclear pore complexes (NPCs) are NE components that regulate nuclear events through interaction with other proteins and DNA. Defects in the nuclear lamina are associated with the development of laminopathies. As cells depleted of phosphoinositide 3-kinase beta (PI3K?) showed an aberrant nuclear morphology, we studied the contribution of PI3K? to maintenance of NE integrity. pik3cb depletion reduced the nuclear membrane tension, triggered formation of areas of lipid bilayer/lamina discontinuity, and impaired NPC assembly. We show that one mechanism for PI3K? regulation of NE/NPC integrity is its association with RCC1 (regulator of chromosome condensation 1), the activator of nuclear Ran GTPase. PI3K? controls RCC1 binding to chromatin and, in turn, Ran activation. These findings suggest that PI3K? regulates the nuclear envelope through upstream regulation of RCC1 and Ran.

SUBMITTER: Redondo-Munoz J 

PROVIDER: S-EPMC4295382 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature