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Antibody-mediated trapping of helminth larvae requires CD11b and Fc? receptor I.

ABSTRACT: Infections with intestinal helminths severely impact on human and veterinary health, particularly through the damage that these large parasites inflict when migrating through host tissues. Host immunity often targets the motility of tissue-migrating helminth larvae, which ideally should be mimicked by anti-helminth vaccines. However, the mechanisms of larval trapping are still poorly defined. We have recently reported an important role for Abs in the rapid trapping of tissue-migrating larvae of the murine parasite Heligmosomoides polygyrus bakeri. Trapping was mediated by macrophages (M?) and involved complement, activating FcRs, and Arginase-1 (Arg1) activity. However, the receptors and Ab isotypes responsible for M? adherence and Arg1 induction remained unclear. Using an in vitro coculture assay of H. polygyrus bakeri larvae and bone marrow-derived M?, we now identify CD11b as the major complement receptor mediating M? adherence to the larval surface. However, larval immobilization was largely independent of CD11b and instead required the activating IgG receptor Fc?RI (CD64) both in vitro and during challenge H. polygyrus bakeri infection in vivo. Fc?RI signaling also contributed to the upregulation of M? Arg1 expression in vitro and in vivo. Finally, IgG2a/c was the major IgG subtype from early immune serum bound by Fc?RI on the M? surface, and purified IgG2c could trigger larval immobilization and Arg1 expression in M? in vitro. Our findings reveal a novel role for IgG2a/c-Fc?RI-driven M? activation in the efficient trapping of tissue-migrating helminth larvae and thus provide important mechanistic insights vital for anti-helminth vaccine development.

SUBMITTER: Esser-von Bieren J

PROVIDER: S-EPMC4298127 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Antibody-mediated trapping of helminth larvae requires CD11b and Fcγ receptor I.

Esser-von Bieren Julia J Volpe Beatrice B Kulagin Manuel M Sutherland Duncan B DB Guiet Romain R Seitz Arne A Marsland Benjamin J BJ Verbeek J Sjef JS Harris Nicola L NL

Journal of immunology (Baltimore, Md. : 1950) 20141229 3

Infections with intestinal helminths severely impact on human and veterinary health, particularly through the damage that these large parasites inflict when migrating through host tissues. Host immunity often targets the motility of tissue-migrating helminth larvae, which ideally should be mimicked by anti-helminth vaccines. However, the mechanisms of larval trapping are still poorly defined. We have recently reported an important role for Abs in the rapid trapping of tissue-migrating larvae of ...[more]

PMID: 25548226

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Project description:C-reactive protein (CRP) is a member of the pentraxin family of proteins. These proteins are highly conserved over the course of evolution being present as far back as 250 million years ago. Mammalian pentraxins are characterized by the presence of five identical non-covalently linked subunits. Each subunit has a structurally conserved site for calcium-dependent ligand binding. The biological activities of the pentraxins established over many years include the ability to mediate opsonization for phagocytosis and complement activation. Pentraxins have an important role in protection from infection from pathogenic bacteria, and regulation of the inflammatory response. It was recognized early on that some of these functions are mediated by activation of the classical complement pathway through C1q. However, experimental evidence suggested that cellular receptors for pentraxins also play a role in phagocytosis. More recent experimental evidence indicates a direct link between pentraxins and Fc receptors. The Fc receptors were first identified as the major receptors for immunoglobulins. The avidity of the interaction between IgG complexes and Fc receptors is greatly enhanced when multivalent ligands interact with the IgG binding sites and activation of signaling pathways requires Fc receptor crosslinking. Human pentraxins bind and activate human and mouse IgG receptors, FcγRI and FcγRII, and the human IgA receptor, FcαRI. The affinities of the interactions between Fc receptors and pentraxins in solution and on cell surfaces are similar to antibody binding to low affinity Fc receptors. Crystallographic and mutagenesis studies have defined the structural features of these interactions and determined the stoichiometry of binding as one-to-one. Pentraxin aggregation or binding to multivalent ligands increases the avidity of binding and results in activation of these receptors for phagocytosis and cytokine synthesis. This review will discuss the structural and functional characteristics of pentraxin Fc receptor interactions and their implications for host defense and inflammation.

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Antibody-mediated trapping of helminth larvae requires CD11b and Fc? receptor I.

Publications

Antibody-mediated trapping of helminth larvae requires CD11b and Fcγ receptor I.

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