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Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. • METHODS AND RESULTS:Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrosequencing without and with library-enrichment methods, resulting in 56,545 sequence reads and 14,467 sequence reads, respectively. All loci showed a high level of polymorphism, with allele numbers ranging from four to 11 in five individuals from five populations (25 samples) and an expected heterozygosity ranging from 0.192 to 0.648 across the loci. • CONCLUSIONS:This set of microsatellite markers is the first one described for A. montana and will facilitate conservation genetic applications as well as the understanding of phylogeographic patterns in this species.

SUBMITTER: Duwe VK

PROVIDER: S-EPMC4298232 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae).

Duwe Virginia K VK Ismail Sascha A SA Buser Andres A Sossai Esther E Borsch Thomas T Muller Ludo A H LA

Applications in plant sciences 20150105 1

<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. •<h4>Methods and results</h4>Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrosequencing without and with library-enrichment methods, resulting in 56,545 sequence reads and 14,467 sequence reads, respectively. All loci showed a high level of polymorp ...[more]

PMID: 25606354

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Project description:Semi-natural mountain grasslands are increasingly exposed to environmental stress under climate change. However, which are the environmental factors that limit plants in these grasslands? Also, is the present management effective against these changes? Fitness-related functional traits may offer a way to detect changes in performance and provide new insights into their vulnerability to climate change. We investigated changes in performance and variability of functional traits of the mountain grassland target species Arnica montana along a climate gradient in Central German low mountain ranges. This gradient represents at its lower end climate conditions that are expected at its upper end under future climate change. We measured vegetative, generative, and physiological traits to account for multiple ways of plant responses to the environment. Using mixed effects and multivariate models, we evaluated changes in trait values among individuals as well as the variability of their populations in order to assess performance under changing summer aridity and different management regimes. Fitness-related performance of most traits showed strongly positive associations with reduced summer aridity at higher elevations, while only specific leaf area and leaf dry matter content showed no association. This suggests a higher performance level at less arid montane sites and that the physiological traits are less sensitive to this climate change factor. The coefficient of variation of almost all traits declined steadily with decreasing site aridity. We suggest that this reduced variability indicates a lower environmental stress level for A. montana toward its environmental optimum at montane elevations, especially because the trait performance increased simultaneously. Surprisingly, management factors and habitat characteristics had only low influence on both trait performance and variability. In summary, summer aridity had a stronger effect to shape the trait performance and variability of A. montana under increased environmental stress than management and other habitat characteristics.

Dataset Information

Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae).

Publications

Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae).

Similar Datasets

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