Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Arnica montana

ABSTRACT: Arnica montana overview

PROVIDER: PRJNA1134825 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR13363423_1.fastq.gz	Fastqsanger.gz
	ERR13363423_2.fastq.gz	Fastqsanger.gz
	ERR13649990.fastq.gz	Fastqsanger.gz
	ERR13999116_1.fastq.gz	Fastqsanger.gz
	ERR13999116_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Arnica montana strain:domestic

Project description:Arnica montana strain:domestic Transcriptome or Gene expression

| PRJNA233448 | ENA

Arnica montana stimulates extracellular matrix gene expression in human macrophages differentiated to wound-healing phenotype.

Project description:Arnica m. effects were associated with a purported anti-inflammatory and tissue healing actions after trauma, bruises, or tissue injuries, but its cellular and molecular mechanisms are largely unknown. Here Arnica m. dilutions were tested using an in vitro model of macrophages polarized towards a “wound-healing” phenotype. The monocyte-macrophage human THP-1 cell line was cultured and differentiated with phorbol-myristate acetate and Interleukin-4, then exposed for 24 h to Arnica m. centesimal (c) dilutions 2c, 3c, 5c,9c, 15c or Control. None of these treatments affected cell viability. A total of 20 genes were differentially expressed comparing cells treated with Arnica m. 2c with those treated with Control only. Of these, 7 genes were up-regulated and 13 were down-regulated. Functional gene enrichment analysis showed that the most significantly upregulated function concerned 4 genes with a conserved site of EGF-like region (p<0.001) and three genes of proteinaceous extracellular matrix, including heparin sulphate proteoglycan 2 (HSPG2), fibrillin 2 (FBN2), and fibronectin (FN1) (p <0.01). Protein assay in supernatants confirmed a statistically significant increase of fibronectin production in Arnica m. 2c treated cells (p<0.05). Pooled extracts of cells treated with increasing dilutions of Arnica m. (3c, 5c, 15c) showed up-regulation of the same group of genes although with lower effect size. The down-regulated transcripts derive from mitochondrial genes coding for some components of electron transport chain. These findings provide new insights into the action of Arnica m. in tissue healing and repair, identifying increased fibronectin production by macrophages as a major therapeutic target.

2016-11-17 | GSE77382 | GEO

Arnica montana stimulates extracellular matrix gene expression in human macrophages differentiated to wound-healing phenotype. Tested on 5 concentrations.

2016-11-17 | GSE77381 | GEO

Arnica mallotopus

Project description:Arnica mallotopus overview

| PRJNA1069827 | ENA

Arnica chamissonis

Project description:Arnica chamissonis Organism overview

| PRJNA84087 | ENA

Arnica mallotopus

Project description:Biogeography of Arnica mallotopus

| PRJDB11419 | ENA