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Sixteen polymorphic microsatellite markers for a federally threatened species, Hexastylis naniflora (Aristolochiaceae), and co-occurring congeners.

ABSTRACT: PREMISE OF THE STUDY:Twenty microsatellite loci were developed for the federally threatened species Hexastylis naniflora (Aristolochiaceae) to examine genetic diversity and to distinguish this species from co-occurring congeners, H. heterophylla and H. minor. METHODS AND RESULTS:Next-generation sequencing approaches were used to identify microsatellite loci and design primers. One hundred fifty-two primer pairs were screened for repeatability, and 20 of these were further characterized for polymorphism. In H. naniflora, the number of alleles identified for polymorphic loci ranged from two to 23 (mean ?8.8), with a mean heterozygosity of 0.39. CONCLUSIONS:These 16 polymorphic primers for H. naniflora will be useful tools in species identification and quantifying genetic diversity within the genus.

SUBMITTER: Hamstead JW 

PROVIDER: S-EPMC4504726 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Sixteen polymorphic microsatellite markers for a federally threatened species, Hexastylis naniflora (Aristolochiaceae), and co-occurring congeners.

Hamstead Jacqueline W JW   Snider Brandon L BL   Oaks Robyn R   Fitzgerald Evan E   Woodward Jason J   Teat Alyssa A   Hay Nikolai M NM   Estep Matt C MC   Murrell Zack E ZE  

Applications in plant sciences 20150707 7

<h4>Premise of the study</h4>Twenty microsatellite loci were developed for the federally threatened species Hexastylis naniflora (Aristolochiaceae) to examine genetic diversity and to distinguish this species from co-occurring congeners, H. heterophylla and H. minor.<h4>Methods and results</h4>Next-generation sequencing approaches were used to identify microsatellite loci and design primers. One hundred fifty-two primer pairs were screened for repeatability, and 20 of these were further characte  ...[more]

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