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Synthesis of bispecific antibodies using genetically encoded unnatural amino acids.

ABSTRACT: Bispecific antibodies were constructed using genetically encoded unnatural amino acids with orthogonal chemical reactivity. A two-step process afforded homogeneous products in excellent yield. Using this approach, we synthesized an anti-HER2/anti-CD3 bispecific antibody, which efficiently cross-linked HER2+ cells and CD3+ cells. In vitro effector-cell mediated cytotoxicity was observed at picomolar concentrations.

SUBMITTER: Kim CH

PROVIDER: S-EPMC4299457 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Synthesis of bispecific antibodies using genetically encoded unnatural amino acids.

Kim Chan Hyuk CH Axup Jun Y JY Dubrovska Anna A Kazane Stephanie A SA Hutchins Benjamin A BA Wold Erik D ED Smider Vaughn V VV Schultz Peter G PG

Journal of the American Chemical Society 20120606 24

Bispecific antibodies were constructed using genetically encoded unnatural amino acids with orthogonal chemical reactivity. A two-step process afforded homogeneous products in excellent yield. Using this approach, we synthesized an anti-HER2/anti-CD3 bispecific antibody, which efficiently cross-linked HER2+ cells and CD3+ cells. In vitro effector-cell mediated cytotoxicity was observed at picomolar concentrations. ...[more]

PMID: 22642368

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