Dataset Information

Prognostic influence of cyclooxygenase-2 protein and mRNA expression in node-negative breast cancer patients.

ABSTRACT:

Background

Cyclooxygenases (COX) play a key role in prostaglandin metabolism and are important for tumor development and progression. The aim of this study was to analyze the prognostic impact of COX-2 expression in a cohort of lymph node-negative breast cancer patients not treated in the adjuvant setting.

Methods

COX-2 expression was determined by immunohistochemistry (IHC) in tumor tissue of 193 node-negative breast cancer patients. Additionally, mRNA expression was determined in corresponding tumor samples using microarray based gene-expression data. Univariate and multivariate Cox regression analyses adjusted for age at diagnosis, tumor size, histological grade, human epithelial growth factor receptor 2 (HER2), estrogen receptor (ER) and progesterone receptor (PR) were performed to evaluate the association of both COX-2 protein and mRNA expression with survival. Survival rates were determined by the Kaplan-Meier method. Correlations between COX-2 expression and established prognostic factors were analyzed using the Chi-square test. A potential correlation between COX-2 protein expression and COX-2 mRNA expression was assessed utilizing the Kruscal-Wallis-H-test.

Results

COX-2 protein expression was positive in 24.9% of the breast cancer samples. Univariate analysis showed that COX-2 protein expression was associated with shorter disease-free survival (DFS) (P = 0.0001), metastasis-free survival (MFS) (P = 0.002) as well as breast cancer specific overall survival (OS) (P = 0.043). In multivariate analysis COX-2 expression retained its significance independent of established prognostic factors for shorter DFS (P < 0.001, HR = 2.767, 95% CI = 1.563-4.901) and for inferior MFS (P = 0.002, HR = 2.7, 95% CI = 1.469-5.263) but not for OS (P = 0.096, HR = 1.929, 95% CI = 0.889-4.187). In contrast, COX-2 mRNA expression was not related to survival and failed to show a correlation with protein expression (P = 0.410).

Conclusions

The present findings support the hypothesis that COX-2 protein but not mRNA expression is associated with an unfavorable outcome in node-negative breast cancer.

SUBMITTER: Sicking I

PROVIDER: S-EPMC4302078 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Publications

Prognostic influence of cyclooxygenase-2 protein and mRNA expression in node-negative breast cancer patients.

Sicking Isabel I Rommens Karlien K Battista Marco J MJ Böhm Daniel D Gebhard Susanne S Lebrecht Antje A Cotarelo Cristina C Hoffmann Gerald G Hengstler Jan G JG Schmidt Marcus M

BMC cancer 20141215

<h4>Background</h4>Cyclooxygenases (COX) play a key role in prostaglandin metabolism and are important for tumor development and progression. The aim of this study was to analyze the prognostic impact of COX-2 expression in a cohort of lymph node-negative breast cancer patients not treated in the adjuvant setting.<h4>Methods</h4>COX-2 expression was determined by immunohistochemistry (IHC) in tumor tissue of 193 node-negative breast cancer patients. Additionally, mRNA expression was determined i ...[more]

PMID: 25511800

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Dataset Information

Prognostic influence of cyclooxygenase-2 protein and mRNA expression in node-negative breast cancer patients.

Background

Methods

Results

Conclusions

Publications

Prognostic influence of cyclooxygenase-2 protein and mRNA expression in node-negative breast cancer patients.

Similar Datasets

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