Ontology highlight
ABSTRACT:
SUBMITTER: Munro JB
PROVIDER: S-EPMC4304640 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20141008 6210
The HIV-1 envelope (Env) mediates viral entry into host cells. To enable the direct imaging of conformational dynamics within Env, we introduced fluorophores into variable regions of the glycoprotein gp120 subunit and measured single-molecule fluorescence resonance energy transfer within the context of native trimers on the surface of HIV-1 virions. Our observations revealed unliganded HIV-1 Env to be intrinsically dynamic, transitioning between three distinct prefusion conformations, whose rela ...[more]