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Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus.

ABSTRACT: The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy-Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.

SUBMITTER: Sousa-Santos C

PROVIDER: S-EPMC4304857 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus.

Sousa-Santos Carla C Fonseca Paulo J PJ Amorim Maria Clara P MC

PeerJ 20150120

The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy-Weinberg equilibrium was found and there was no evidence for linkage disequili ...[more]

PMID: 25653909

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