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A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting.


ABSTRACT: Integrin activation, which is regulated by allosteric changes in receptor conformation, enables cellular responses to the chemical, mechanical and topological features of the extracellular microenvironment. A global view of how activation state converts the molecular composition of the region proximal to integrins into functional readouts is, however, lacking. Here, using conformation-specific monoclonal antibodies, we report the isolation of integrin activation state-dependent complexes and their characterization by mass spectrometry. Quantitative comparisons, integrating network, clustering, pathway and image analyses, define multiple functional protein modules enriched in a conformation-specific manner. Notably, active integrin complexes are specifically enriched for proteins associated with microtubule-based functions. Visualization of microtubules on micropatterned surfaces and live cell imaging demonstrate that active integrins establish an environment that stabilizes microtubules at the cell periphery. These data provide a resource for the interrogation of the global molecular connections that link integrin activation to adhesion signalling.

SUBMITTER: Byron A 

PROVIDER: S-EPMC4317495 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting.

Byron Adam A   Askari Janet A JA   Humphries Jonathan D JD   Jacquemet Guillaume G   Koper Ewa J EJ   Warwood Stacey S   Choi Colin K CK   Stroud Matthew J MJ   Chen Christopher S CS   Knight David D   Humphries Martin J MJ  

Nature communications 20150122


Integrin activation, which is regulated by allosteric changes in receptor conformation, enables cellular responses to the chemical, mechanical and topological features of the extracellular microenvironment. A global view of how activation state converts the molecular composition of the region proximal to integrins into functional readouts is, however, lacking. Here, using conformation-specific monoclonal antibodies, we report the isolation of integrin activation state-dependent complexes and the  ...[more]

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