Ontology highlight
ABSTRACT:
SUBMITTER: Conti BJ
PROVIDER: S-EPMC4351553 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Conti Brian J BJ Elferich Johannes J Yang Zhongying Z Shinde Ujwal U Skach William R WR
Nature structural & molecular biology 20140223 3
Eukaryotic secretory proteins cross the endoplasmic reticulum (ER) membrane through a protein-conducting channel contained within the ribosome-Sec61translocon complex (RTC). Using a zinc-finger sequence as a folding switch, we show that cotranslational folding of a secretory passenger inhibits translocation in canine ER microsomes and in human cells. Folding occurs within a cytosolically inaccessible environment, after ER targeting but before initiation of translocation, and it is most effective ...[more]