Unknown

Dataset Information

0

Development of molecular markers tightly linked to Pvr4 gene in pepper using next-generation sequencing.


ABSTRACT: It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. Potyvirus resistance 4 (Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to Pvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and map Pvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site-leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes.

SUBMITTER: Devran Z 

PROVIDER: S-EPMC4361654 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4666610 | biostudies-literature
| S-EPMC4873275 | biostudies-literature
| S-EPMC4683043 | biostudies-literature
| S-EPMC5664088 | biostudies-literature
| S-EPMC4109682 | biostudies-other
| S-EPMC4865750 | biostudies-literature
| S-EPMC3431879 | biostudies-literature
| S-EPMC4575593 | biostudies-literature
| S-EPMC5584814 | biostudies-literature
| S-EPMC6240451 | biostudies-literature