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Development of 14 microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus) using next generation sequencing technology.

ABSTRACT: We report the development of 14 new microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus). Ten unrelated Queensland koala individuals from the San Diego Zoo, USA, were genotyped. The number of alleles per locus ranged from 2 to 7, with an average of 5.14 alleles per locus. Across all loci, the average observed and expected heterozygosity values were both 0.69. These polymorphic microsatellite loci will be useful for genetic studies relevant to the conservation of the koala, a species listed as vulnerable.

SUBMITTER: Ruiz-Rodriguez CT

PROVIDER: S-EPMC4109682 | biostudies-other | 2014 Jun

REPOSITORIES: biostudies-other

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Development of 14 microsatellite markers in the Queensland koala (<i>Phascolarctos cinereus adustus</i>) using next generation sequencing technology.

Ruiz-Rodriguez Christina T CT Ishida Yasuko Y Greenwood Alex D AD Roca Alfred L AL

Conservation genetics resources 20140601 2

We report the development of 14 new microsatellite markers in the Queensland koala (<i>Phascolarctos cinereus adustus</i>). Ten unrelated Queensland koala individuals from the San Diego Zoo, USA, were genotyped. The number of alleles per locus ranged from 2 to 7, with an average of 5.14 alleles per locus. Across all loci, the average observed and expected heterozygosity values were both 0.69. These polymorphic microsatellite loci will be useful for genetic studies relevant to the conservation of ...[more]

PMID: 25067980

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