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Development of 17 novel microsatellite markers for Lycoris aurea and L. radiata (Amaryllidaceae) using next-generation sequencing.

ABSTRACT: Premise of the Study:Lycoris is an ornamental and medicinal plant. We developed microsatellite markers for L. aurea and L. radiata simultaneously by using a hybrid between these two species. Methods and Results:Ion Torrent next-generation sequencing produced 1,784,504 reads. Testing 64 primer sets allowed for the development of 17 novel microsatellite markers: 16 for L. aurea, 10 for L. radiata, and nine common markers. Lycoris aurea had one to 12 alleles per locus and observed and expected heterozygosity levels of 0-0.923 and 0.038-0.809, respectively. Lycoris radiata had three to 12 alleles per locus and observed and expected heterozygosity levels of 0-0.909 and 0.127-0.797, respectively. Ten markers were cross-amplified for L. sprengeri. Conclusions:Hybrid sequencing can facilitate the cost-effective development of molecular markers for parental species. The markers developed here are useful for studying Lycoris population structure.

SUBMITTER: Chen IJ 

PROVIDER: S-EPMC6240451 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Development of 17 novel microsatellite markers for <i>Lycoris aurea</i> and <i>L. radiata</i> (Amaryllidaceae) using next-generation sequencing.

Chen I-Ju IJ   Shii Chou-Tou CT   Chang Tsu-Liang TL   Hwu Kae-Kang KK  

Applications in plant sciences 20181114 11

<h4>Premise of the study</h4><i>Lycoris</i> is an ornamental and medicinal plant. We developed microsatellite markers for <i>L. aurea</i> and <i>L. radiata</i> simultaneously by using a hybrid between these two species.<h4>Methods and results</h4>Ion Torrent next-generation sequencing produced 1,784,504 reads. Testing 64 primer sets allowed for the development of 17 novel microsatellite markers: 16 for <i>L. aurea</i>, 10 for <i>L. radiata</i>, and nine common markers. <i>Lycoris aurea</i> had o  ...[more]

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