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Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE).

ABSTRACT: One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.

SUBMITTER: Deutsch EW 

PROVIDER: S-EPMC4367930 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE).

Deutsch Eric W EW   Ball Catherine A CA   Berman Jules J JJ   Bova G Steven GS   Brazma Alvis A   Bumgarner Roger E RE   Campbell David D   Causton Helen C HC   Christiansen Jeffrey H JH   Daian Fabrice F   Dauga Delphine D   Davidson Duncan R DR   Gimenez Gregory G   Goo Young Ah YA   Grimmond Sean S   Henrich Thorsten T   Herrmann Bernhard G BG   Johnson Michael H MH   Korb Martin M   Mills Jason C JC   Oudes Asa J AJ   Parkinson Helen E HE   Pascal Laura E LE   Pollet Nicolas N   Quackenbush John J   Ramialison Mirana M   Ringwald Martin M   Salgado David D   Sansone Susanna-Assunta SA   Sherlock Gavin G   Stoeckert Christian J CJ   Swedlow Jason J   Taylor Ronald C RC   Walashek Laura L   Warford Anthony A   Wilkinson David G DG   Zhou Yi Y   Zon Leonard I LI   Liu Alvin Y AY   True Lawrence D LD  

Nature biotechnology 20080301 3

One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experim  ...[more]

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