Ontology highlight
ABSTRACT:
SUBMITTER: Hunter RW
PROVIDER: S-EPMC4370744 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Hunter Roger W RW Zeqiraj Elton E Morrice Nicholas N Sicheri Frank F Sakamoto Kei K
Protein expression and purification 20141217
We report the successful expression and purification of functional human muscle glycogen synthase (GYS1) in complex with human glycogenin-1 (GN1). Stoichiometric GYS1:GN1 complex was produced by co-expression of GYS1 and GN1 using a bicistronic pFastBac™-Dual expression vector, followed by affinity purification and subsequent size-exclusion chromatography. Mass spectrometry analysis identified that GYS1 is phosphorylated at several well-characterised and uncharacterised Ser/Thr residues. Biochem ...[more]