Macrophage gene expression associated with remodeling of the prepartum rat cervix: microarray and pathway analyses.

ABSTRACT: As the critical gatekeeper for birth, prepartum remodeling of the cervix is associated with increased resident macrophages (M?), proinflammatory processes, and extracellular matrix degradation. This study tested the hypothesis that expression of genes unique to M?s characterizes the prepartum from unremodeled nonpregnant cervix. Perfused cervix from prepartum day 21 postbreeding (D21) or nonpregnant (NP) rats, with or without M?s, had RNA extracted and whole genome microarray analysis performed. By subtractive analyses, expression of 194 and 120 genes related to M?s in the cervix from D21 rats were increased and decreased, respectively. In both D21 and NP groups, 158 and 57 M? genes were also more or less up- or down-regulated, respectively. M? gene expression patterns were most strongly correlated within groups and in 5 major clustering patterns. In the cervix from D21 rats, functional categories and canonical pathways of increased expression by M? gene related to extracellular matrix, cell proliferation, differentiation, as well as cell signaling. Pathways were characteristic of inflammation and wound healing, e.g., CD163, CD206, and CCR2. Signatures of only inflammation pathways, e.g., CSF1R, EMR1, and MMP12 were common to both D21 and NP groups. Thus, a novel and complex balance of M? genes and clusters differentiated the degraded extracellular matrix and cellular genomic activities in the cervix before birth from the unremodeled state. Predicted M? activities, pathways, and networks raise the possibility that expression patterns of specific genes characterize and promote prepartum remodeling of the cervix for parturition at term and with preterm labor.

SUBMITTER: Dobyns AE 

PROVIDER: S-EPMC4374766 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature