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Comparative quantification of the surfaceome of human multipotent mesenchymal progenitor cells.


ABSTRACT: Mesenchymal progenitor cells have great therapeutic potential, yet incomplete characterization of their cell-surface interface limits their clinical exploitation. We have employed subcellular fractionation with quantitative discovery proteomics to define the cell-surface interface proteome of human bone marrow mesenchymal stromal/stem cells (MSCs) and human umbilical cord perivascular cells (HUCPVCs). We compared cell-surface-enriched fractions from MSCs and HUCPVCs (three donors each) with adult mesenchymal fibroblasts using eight-channel isobaric-tagging mass spectrometry, yielding relative quantification on >6,000 proteins with high confidence. This approach identified 186 upregulated mesenchymal progenitor biomarkers. Validation of 10 of these markers, including ROR2, EPHA2, and PLXNA2, confirmed upregulated expression in mesenchymal progenitor populations and distinct roles in progenitor cell proliferation, migration, and differentiation. Our approach has delivered a cell-surface proteome repository that now enables improved selection and characterization of human mesenchymal progenitor populations.

SUBMITTER: Holley RJ 

PROVIDER: S-EPMC4375938 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Comparative quantification of the surfaceome of human multipotent mesenchymal progenitor cells.

Holley Rebecca J RJ   Tai Guangping G   Williamson Andrew J K AJ   Taylor Samuel S   Cain Stuart A SA   Richardson Stephen M SM   Merry Catherine L R CL   Whetton Anthony D AD   Kielty Cay M CM   Canfield Ann E AE  

Stem cell reports 20150213 3


Mesenchymal progenitor cells have great therapeutic potential, yet incomplete characterization of their cell-surface interface limits their clinical exploitation. We have employed subcellular fractionation with quantitative discovery proteomics to define the cell-surface interface proteome of human bone marrow mesenchymal stromal/stem cells (MSCs) and human umbilical cord perivascular cells (HUCPVCs). We compared cell-surface-enriched fractions from MSCs and HUCPVCs (three donors each) with adul  ...[more]

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