Ontology highlight
ABSTRACT:
SUBMITTER: Wang J
PROVIDER: S-EPMC4379447 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Wang Ji J Place Robert F RF Portnoy Victoria V Huang Vera V Kang Moo Rim MR Kosaka Mika M Ho Maurice Kwok Chung MKC Li Long-Cheng LC
Journal of biological methods 20150301 1
Vector-based systems comprised of exogenous nucleic acid sequences remain the standard for ectopic expression of a particular gene. Such systems offer robust overexpression, but have inherent drawbacks such as the tedious process of construction, excluding sequences (e.g. introns and untranslated regions) important for gene function and potential insertional mutagenesis of host genome associated with the use of viral vectors. We and others have recently reported that short double-stranded RNAs ( ...[more]