Altered imprinted gene expression and methylation patterns in mid-gestation aborted cloned porcine fetuses and placentas.

ABSTRACT:

Purpose

To determine the expression patterns of imprinted genes and their methylation status in aborted cloned porcine fetuses and placentas.

Methods

RNA and DNA were prepared from fetuses and placentas that were produced by SCNT and controls from artificial insemination. The expression of 18 imprinted genes was determined by quantitative real-time PCR (q-PCR). Bisulfite sequencing PCR (BSP) was conducted to determine the methylation status of PRE-1 short interspersed repetitive element (SINE), satellite DNA and H19 differentially methylated region 3 (DMR3).

Results

The weight, imprinted gene expression and genome-wide DNA methylation patterns were compared between the mid-gestation aborted and normal control samples. The results showed hypermethylation of PRE-1 and s

SUBMITTER: Zhang X 

PROVIDER: S-EPMC4389936 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature