Genome-wide identification of FoxO-dependent gene networks in skeletal muscle during C26 cancer cachexia.

ABSTRACT:

Background

Evidence from cachectic cancer patients and animal models of cancer cachexia supports the involvement of Forkhead box O (FoxO) transcription factors in driving cancer-induced skeletal muscle wasting. However, the genome-wide gene networks and associated biological processes regulated by FoxO during cancer cachexia are unknown. We hypothesize that FoxO is a central upstream regulator of diverse gene networks in skeletal muscle during cancer that may act coordinately to promote the wasting phenotype.

Methods

To inhibit endogenous FoxO DNA-binding, we transduced limb and diaphragm muscles of mice with AAV9 containing the cDNA for a dominant negative (d.n.) FoxO protein (or GFP control). The d.n.FoxO construct consists of only the FoxO3a DNA-binding domain that is hig

SUBMITTER: Judge SM 

PROVIDER: S-EPMC4391468 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature