Unknown

Dataset Information

0

The p53 isoform ?133p53? promotes cancer stem cell potential.

ABSTRACT: Cancer stem cells (CSC) are responsible for cancer chemoresistance and metastasis formation. Here we report that ?133p53?, a TP53 splice variant, enhanced cancer cell stemness in MCF-7 breast cancer cells, while its depletion reduced it. ?133p53? stimulated the expression of the key pluripotency factors SOX2, OCT3/4, and NANOG. Similarly, in highly metastatic breast cancer cells, aggressiveness was coupled with enhanced CSC potential and ?133p53? expression. Like in MCF-7 cells, SOX2, OCT3/4, and NANOG expression were positively regulated by ?133p53? in these cells. Finally, treatment of MCF-7 cells with etoposide, a cytotoxic anti-cancer drug, increased CSC formation and SOX2, OCT3/4, and NANOG expression via ?133p53, thus potentially increasing the risk of cancer recurrence. Our findings show that ?133p53? supports CSC potential. Moreover, they indicate that the TP53 gene, which is considered a major tumor suppressor gene, also acts as an oncogene via the ?133p53? isoform.

SUBMITTER: Arsic N 

PROVIDER: S-EPMC4400643 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Cancer stem cells (CSC) are responsible for cancer chemoresistance and metastasis formation. Here we report that Δ133p53β, a TP53 splice variant, enhanced cancer cell stemness in MCF-7 breast cancer cells, while its depletion reduced it. Δ133p53β stimulated the expression of the key pluripotency factors SOX2, OCT3/4, and NANOG. Similarly, in highly metastatic breast cancer cells, aggressiveness was coupled with enhanced CSC potential and Δ133p53β expression. Like in MCF-7 cells, SOX2, OCT3/4, an  ...[more]

Similar Datasets

Unknown The ?133p53? isoform promotes an immunosuppressive environment leading to aggressive prostate cancer.
| S-EPMC6702175 | biostudies-literature
Unknown Elevation of the TP53 isoform ?133p53? in glioblastomas: an alternative to mutant p53 in promoting tumor development.
| S-EPMC6120556 | biostudies-literature
Unknown The RNA helicase p68 modulates expression and function of the ?133 isoform(s) of p53, and is inversely associated with ?133p53 expression in breast cancer.
| S-EPMC3016604 | biostudies-literature
Unknown ∆133p53 isoform promotes tumour invasion and metastasis via interleukin-6 activation of JAK-STAT and RhoA-ROCK signalling.
| S-EPMC5772473 | biostudies-literature
Unknown Regulation of the interferon-gamma (IFN-?) pathway by p63 and ?133p53 isoform in different breast cancer subtypes.
| S-EPMC6044385 | biostudies-literature
Unknown p53 isoform Δ133p53 promotes efficiency of induced pluripotent stem cells and ensures genomic integrity during reprogramming.
| S-EPMC5118801 | biostudies-other
Unknown p53 isoform expression promotes a stemness phenotype and inhibits doxorubicin sensitivity in breast cancer.
| S-EPMC10409720 | biostudies-literature
Unknown The p53 isoform delta133p53ß regulates cancer cell apoptosis in a RhoB-dependent manner.
| S-EPMC5315499 | biostudies-literature
Unknown Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.
| S-EPMC8773822 | biostudies-literature
Unknown CD44 splice isoform switching determines breast cancer stem cell state.
| S-EPMC6362815 | biostudies-literature