Blocking IKK? expression inhibits prostate cancer invasiveness.

ABSTRACT: IKK? has been recently identified as a key mediator of the inflammation and metastasis in prostate cancer. In the present study, we intend to silence the IKK? expression in prostate cancer cells using synthetic siRNAs and examine their biological effects on tumor cell invasiveness and growth.Three synthetic siRNAs targeting different regions of the IKK? mRNA were designed, and the silencing effect was determined in PC-3 and DU145 cells. Numerous studies, including wound-healing assay, migration assay, invasion assay, cell attachment assay, cell proliferation, and cell cycle analysis, were conducted to investigate the biological effects of the IKK? siRNAs on prostate cancer cells.We have identified potent siRNAs that can silence the IKK? up to 74%. Inhibition of IKK? reduced the wound healing, migration, invasion and cell attachment capabilities of prostate cancer cells. Similar anti-invasive effects were also observed in the presence of RANKL. However, silencing of IKK? only showed a negligible effect on cell proliferation and cell cycle distribution.This study presents compelling evidence that IKK? plays a major role in prostate cancer invasion and metastasis, but not in cell proliferation. Silencing of IKK? with siRNA may therefore provide a promising therapeutic strategy for prostate cancer patients.

SUBMITTER: Mahato R 

PROVIDER: S-EPMC4406877 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature