Ontology highlight
ABSTRACT:
SUBMITTER: Thorsen TS
PROVIDER: S-EPMC4408211 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Thorsen Thor Seneca TS Matt Rachel R Weis William I WI Kobilka Brian K BK
Structure (London, England : 1993) 20141023 11
G protein-coupled receptors (GPCRs) mediate the majority of cellular responses to hormones and neurotransmitters. Most GPCR crystal structures have been obtained using a fusion protein strategy where the flexible third intracellular loop is replaced by T4 lysozyme (T4L). However, wild-type T4L may not be ideally suited for all GPCRs because of its size and the inherent flexibility between the N- and C-terminal subdomains. Here we report two modified T4L variants, designed to address flexibility ...[more]