Ontology highlight
ABSTRACT:
SUBMITTER: Soskine M
PROVIDER: S-EPMC4410319 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Soskine Misha M Biesemans Annemie A De Maeyer Marc M Maglia Giovanni G
Journal of the American Chemical Society 20130827 36
Nanopores have recently emerged as powerful tools in single-molecule investigations. Biological nanopores, however, have drawbacks, including a fixed size and limited stability in lipid bilayers. Inspired by the great success of directed evolution approaches in tailoring enzyme properties, in this work we evolved Cytolysin A from Salmonella typhi (ClyA) to a high level of soluble expression and desired electrical properties in lipid bilayers. Evolved ClyA nanopores remained open up to -150 mV ap ...[more]