Ontology highlight
ABSTRACT:
SUBMITTER: Fedorova O
PROVIDER: S-EPMC4419380 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Fedorova Olga O Pyle Anna Marie AM
Journal of molecular biology 20120613 3
The ai5γ group II intron requires a protein cofactor to facilitate native folding in the cell. Yeast protein Mss116 greatly accelerates intron folding under near-physiological conditions both in vivo and in vitro. Although the effect of Mss116 on the kinetics of ai5γ ribozyme folding and catalysis has been extensively studied, the precise structural role and interaction sites of Mss116 have been elusive. Using Nucleotide Analog Interference Mapping to study the folding of splicing precursor cons ...[more]