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Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR.


ABSTRACT: By applying [1-(13) C]- and [2-(13) C]-glucose labeling schemes to the folded globular protein ubiquitin, a strong reduction of spectral crowding and increase in resolution in solid-state NMR (ssNMR) spectra could be achieved. This allowed spectral resonance assignment in a straightforward manner and the collection of a wealth of long-range distance information. A high precision solid-state NMR structure of microcrystalline ubiquitin was calculated with a backbone rmsd of 1.57 to the X-ray structure and 1.32 Å to the solution NMR structure. Interestingly, we can resolve structural heterogeneity as the presence of three slightly different conformations. Structural heterogeneity is most significant for the loop region ?1-?2 but also for ?-strands ?1, ?2, ?3, and ?5 as well as for the loop connecting ?1 and ?3. This structural polymorphism observed in the solid-state NMR spectra coincides with regions that showed dynamics in solution NMR experiments on different timescales.

SUBMITTER: Fasshuber HK 

PROVIDER: S-EPMC4420510 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR.

Fasshuber Hannes Klaus HK   Lakomek Nils-Alexander NA   Habenstein Birgit B   Loquet Antoine A   Shi Chaowei C   Giller Karin K   Wolff Sebastian S   Becker Stefan S   Lange Adam A  

Protein science : a publication of the Protein Society 20150316 5


By applying [1-(13) C]- and [2-(13) C]-glucose labeling schemes to the folded globular protein ubiquitin, a strong reduction of spectral crowding and increase in resolution in solid-state NMR (ssNMR) spectra could be achieved. This allowed spectral resonance assignment in a straightforward manner and the collection of a wealth of long-range distance information. A high precision solid-state NMR structure of microcrystalline ubiquitin was calculated with a backbone rmsd of 1.57 to the X-ray struc  ...[more]

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