Ontology highlight
ABSTRACT:
SUBMITTER: Madrzak J
PROVIDER: S-EPMC4423210 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Madrzak Julia J Fiedler Marc M Johnson Christopher M CM Ewan Richard R Knebel Axel A Bienz Mariann M Chin Jason W JW
Nature communications 20150424
Dishevelled relays Wnt signals from the plasma membrane to different cytoplasmic effectors. Its signalling activity depends on its DIX domain, which undergoes head-to-tail polymerization to assemble signalosomes. The DIX domain is ubiquitinated in vivo at multiple lysines, which can be antagonized by various deubiquitinases (DUBs) including the CYLD tumour suppressor that attenuates Wnt signalling. Here, we generate milligram quantities of pure human Dvl2 DIX domain mono-ubiquitinated at two lys ...[more]