Ontology highlight
ABSTRACT:
SUBMITTER: Cowley GS
PROVIDER: S-EPMC4432652 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Scientific data 20140930
Using a genome-scale, lentivirally delivered shRNA library, we performed massively parallel pooled shRNA screens in 216 cancer cell lines to identify genes that are required for cell proliferation and/or viability. Cell line dependencies on 11,000 genes were interrogated by 5 shRNAs per gene. The proliferation effect of each shRNA in each cell line was assessed by transducing a population of 11M cells with one shRNA-virus per cell and determining the relative enrichment or depletion of each of t ...[more]