Ontology highlight
ABSTRACT:
SUBMITTER: Kondabagil K
PROVIDER: S-EPMC4438746 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Virology 20141016
The packaging motor of bacteriophage T4 translocates DNA into the capsid at a rate of up to 2000 bp/s. Such a high rate would require coordination of motor movements at millisecond timescale. Designing a cysteine-less gp17 is essential to generate fluorescently labeled motors and measure distance changes between motor domains by FRET analyses. Here, by using sequence alignments, structural modeling, combinatorial mutagenesis, and recombinational rescue, we replaced all nine cysteines of gp17 and ...[more]