Analysis by live imaging of effects of the adenovirus E4orf4 protein on passage through mitosis of H1299 tumor cells.
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ABSTRACT:
SUBMITTER: Sriskandarajah N
PROVIDER: S-EPMC4442376 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Publications
Analysis by live imaging of effects of the adenovirus E4orf4 protein on passage through mitosis of H1299 tumor cells.
Sriskandarajah Neera N Blanchette Paola P Kucharski Thomas J TJ Teodoro Jose G JG Branton Philip E PE
Journal of virology 20150204 8
The adenovirus E4orf4 protein expressed at high levels kills cancer cells but not normal human primary cells. Previous studies suggested that disruption of processes that regulate mitosis may underlie E4orf4 toxicity. Here we have used live imaging to show that E4orf4 induces a slowed defective transit through mitosis, exhibiting a delay or often failure in cytokinesis that may account for an accumulation of G1 tetraploids in the population of dying E4orf4-expressing cells. ...[more]
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