Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis.

ABSTRACT: When human cells enter mitosis, chromosomes undergo substantial changes in their organization to resolve sister chromatids and compact chromosomes. To comprehend the timing and coordination of these events, we need to evaluate the progression of both sister chromatid resolution and chromosome compaction in one assay. Here we achieved this by analyzing changes in configuration of marked chromosome regions over time, with high spatial and temporal resolution. This assay showed that sister chromatids cycle between nonresolved and partially resolved states with an interval of a few minutes during G2 phase before completing full resolution in prophase. Cohesins and WAPL antagonistically regulate sister chromatid resolution in late G2 and prophase while local enrichment of cohesin on chromosomes

SUBMITTER: Eykelenboom JK 

PROVIDER: S-EPMC6504890 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature