Ontology highlight
ABSTRACT:
SUBMITTER: Lu Y
PROVIDER: S-EPMC4449139 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20150409 6231
Biological processes require specific enzymatic reactions, paradoxically involving short recognition sequences. As an example, cell-cycle timing depends on a sequence of ubiquitylation events mediated by the anaphase-promoting complex (APC) based on short redundant motifs. To understand the origin of specificity, we designed single-molecule fluorescence assays that capture transient ubiquitylation reactions. We find that the APC-mediated ubiquitylation involves a highly processive initial reacti ...[more]