Determinants for degradation of SAMHD1, Mus81 and induction of G2 arrest in HIV-1 Vpr and SIVagm Vpr.
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ABSTRACT:
SUBMITTER: DePaula-Silva AB
PROVIDER: S-EPMC4455942 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Determinants for degradation of SAMHD1, Mus81 and induction of G2 arrest in HIV-1 Vpr and SIVagm Vpr.
DePaula-Silva Ana Beatriz AB Cassiday Patrick A PA Chumley Jeffrey J Bosque Alberto A Monteiro-Filho Carlos M R CMR Mahon Cathal S CS Cone Kelsey R KR Krogan Nevan N Elde Nels C NC Planelles Vicente V
Virology 20150122
Vpr and Vpx are a group of highly related accessory proteins from primate lentiviruses. Despite the high degree of amino acid homology within this group, these proteins can be highly divergent in their functions. In this work, we constructed chimeric and mutant proteins between HIV-1 and SIVagm Vpr in order to better understand the structure-function relationships. We tested these constructs for their abilities to induce G2 arrest in human cells and to degrade agmSAMHD1 and Mus81. We found that ...[more]
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