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Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters.

ABSTRACT: We present an attolitre-sized arrayed lipid bilayer chamber system (aL-ALBiC) for rapid and massively parallel single-molecule assay of membrane transporter activity. Because of the small reaction volume (200?aL), the aL-ALBiC performed fast detection of single transporter activity, thereby enhancing the sensitivity, throughput, and accuracy of the analysis. Thus, aL-ALBiC broadens the opportunities for single-molecule analysis of various membrane transporters and can be used in pharmaceutical applications such as drug screening.

SUBMITTER: Soga N

PROVIDER: S-EPMC4458879 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters.

Soga Naoki N Watanabe Rikiya R Noji Hiroyuki H

Scientific reports 20150608

We present an attolitre-sized arrayed lipid bilayer chamber system (aL-ALBiC) for rapid and massively parallel single-molecule assay of membrane transporter activity. Because of the small reaction volume (200 aL), the aL-ALBiC performed fast detection of single transporter activity, thereby enhancing the sensitivity, throughput, and accuracy of the analysis. Thus, aL-ALBiC broadens the opportunities for single-molecule analysis of various membrane transporters and can be used in pharmaceutical a ...[more]

PMID: 26052065

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