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Pyrrole-imidazole polyamide targeted to break fusion sites in TMPRSS2 and ERG gene fusion represses prostate tumor growth.

ABSTRACT: Aberrant overexpression of ERG induced by the TMPRSS2-ERG gene fusion is likely involved in the development of prostate cancer. Synthetic pyrrole-imidazole (PI) polyamides recognize and attach to the minor groove of DNA with high affinity and specificity. In the present study, we designed a PI polyamide targeting TMPRSS2-ERG translocation breakpoints and assessed its effect on human prostate cancer cells. Our study identified that this PI polyamide repressed the cell and tumor growth of androgen-sensitive LNCaP prostate cancer cells. Targeting of these breakpoint sequences by PI polyamides could be a novel approach for the treatment of prostate cancer.

SUBMITTER: Obinata D

PROVIDER: S-EPMC4462350 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Pyrrole-imidazole polyamide targeted to break fusion sites in TMPRSS2 and ERG gene fusion represses prostate tumor growth.

Obinata Daisuke D Ito Akiko A Fujiwara Kyoko K Takayama Ken-Ichi K Ashikari Daisaku D Murata Yasutaka Y Yamaguchi Kenya K Urano Tomohiko T Fujimura Tetsuya T Fukuda Noboru N Soma Masayoshi M Watanabe Takayoshi T Nagase Hiroki H Inoue Satoshi S Takahashi Satoru S

Cancer science 20140918 10

Aberrant overexpression of ERG induced by the TMPRSS2-ERG gene fusion is likely involved in the development of prostate cancer. Synthetic pyrrole-imidazole (PI) polyamides recognize and attach to the minor groove of DNA with high affinity and specificity. In the present study, we designed a PI polyamide targeting TMPRSS2-ERG translocation breakpoints and assessed its effect on human prostate cancer cells. Our study identified that this PI polyamide repressed the cell and tumor growth of androgen ...[more]

PMID: 25088707

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