Unknown

Dataset Information

0

In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues.


ABSTRACT: The invention of peptide-MHC-tetramer technology to label antigen-specific T cells has led to an enhanced understanding of T lymphocyte biology. Here we describe the development of an in situ pMHC-II tetramer staining method to visualize antigen-specific CD4+ T cells in tissues. This method complements other methods developed that similarly use MHC class II reagents to stain antigen-specific CD4+ T cells in situ. In this study, we used group A streptococcus (GAS) expressing a surrogate peptide (2W) to inoculate C57BL/6 mice, and used fresh nasal-associated lymphoid tissues (NALT) in optimizing the in situ staining of 2W:I-Ab specific CD4+ T cells. The results showed 2W:I-Ab tetramer-binding CD4+ T cells in GAS-2W but not GAS infected mice. This method holds promise to be broadly applicable to study the localization, abundance, and phenotype of antigen-specific CD4+ T cells in undisrupted tissues.

SUBMITTER: Dileepan T 

PROVIDER: S-EPMC4465905 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues.

Dileepan Thamotharampillai T   Kim Hyeon O HO   Cleary P Patrick PP   Skinner Pamela J PJ  

PloS one 20150611 6


The invention of peptide-MHC-tetramer technology to label antigen-specific T cells has led to an enhanced understanding of T lymphocyte biology. Here we describe the development of an in situ pMHC-II tetramer staining method to visualize antigen-specific CD4+ T cells in tissues. This method complements other methods developed that similarly use MHC class II reagents to stain antigen-specific CD4+ T cells in situ. In this study, we used group A streptococcus (GAS) expressing a surrogate peptide (  ...[more]

Similar Datasets

| S-EPMC8802177 | biostudies-literature
| S-EPMC4671089 | biostudies-literature
| S-EPMC5013714 | biostudies-literature
| S-EPMC5006925 | biostudies-literature
| S-EPMC3511074 | biostudies-literature
| S-EPMC3085167 | biostudies-literature
| S-EPMC10666075 | biostudies-literature
| S-EPMC3510669 | biostudies-literature
| S-EPMC2975033 | biostudies-literature
| S-EPMC2867704 | biostudies-literature